Effects of Brazilian and Bulgarian propolis on bactericidal activity of macrophages against Salmonella Typhimurium.
Identifieur interne : 004055 ( Main/Exploration ); précédent : 004054; suivant : 004056Effects of Brazilian and Bulgarian propolis on bactericidal activity of macrophages against Salmonella Typhimurium.
Auteurs : Ricardo O. Orsi [Brésil] ; José M. Sforcin ; Sílvia R C. Funari ; Vassya BankovaSource :
- International immunopharmacology [ 1567-5769 ] ; 2005.
Descripteurs français
- KwdFr :
- Activation des macrophages (effets des médicaments et des substances chimiques), Activation des macrophages (immunologie), Animaux (MeSH), Brésil (MeSH), Bulgarie (MeSH), Cellules cultivées (MeSH), Facteurs immunologiques (pharmacologie), Macrophages péritonéaux (effets des médicaments et des substances chimiques), Macrophages péritonéaux (immunologie), Macrophages péritonéaux (microbiologie), Mâle (MeSH), Propolis (pharmacologie), Salmonella typhimurium (croissance et développement), Salmonella typhimurium (immunologie), Souris (MeSH), Souris de lignée BALB C (MeSH).
- MESH :
- croissance et développement : Salmonella typhimurium.
- effets des médicaments et des substances chimiques : Activation des macrophages, Macrophages péritonéaux.
- immunologie : Activation des macrophages, Macrophages péritonéaux, Salmonella typhimurium.
- microbiologie : Macrophages péritonéaux.
- pharmacologie : Facteurs immunologiques, Propolis.
- Animaux, Brésil, Bulgarie, Cellules cultivées, Mâle, Souris, Souris de lignée BALB C.
- Wicri :
English descriptors
- KwdEn :
- Animals (MeSH), Brazil (MeSH), Bulgaria (MeSH), Cells, Cultured (MeSH), Immunologic Factors (pharmacology), Macrophage Activation (drug effects), Macrophage Activation (immunology), Macrophages, Peritoneal (drug effects), Macrophages, Peritoneal (immunology), Macrophages, Peritoneal (microbiology), Male (MeSH), Mice (MeSH), Mice, Inbred BALB C (MeSH), Propolis (pharmacology), Salmonella typhimurium (growth & development), Salmonella typhimurium (immunology).
- MESH :
- chemical , pharmacology : Immunologic Factors, Propolis.
- geographic : Brazil, Bulgaria.
- drug effects : Macrophage Activation, Macrophages, Peritoneal.
- growth & development : Salmonella typhimurium.
- immunology : Macrophage Activation, Macrophages, Peritoneal, Salmonella typhimurium.
- microbiology : Macrophages, Peritoneal.
- Animals, Cells, Cultured, Male, Mice, Mice, Inbred BALB C.
Abstract
Propolis has been used in folk medicine since ancient times due to its many biological properties, such as antimicrobial, antiinflammatory, antioxidant, immunomodulatory activities, among others. Macrophages play an important role in the early phase of Salmonella infection. In this work, macrophages were prestimulated with Brazilian or Bulgarian propolis and subsequently challenged with Salmonella Typhimurium at different macrophage/bacteria ratio. After 60 min of incubation, cells were harvested with Triton-X to lyse the macrophages. To assess the bactericidal activity, the number of colony-forming units (CFU) of S. typhimurium was determined by plating 0.1 mL in Mueller Hinton agar. After 24 h, CFU were counted, and the percentage of bactericidal activity was obtained. Propolis from Brazil and Bulgaria enhanced the bactericidal activity of macrophages, depending on its concentration. Brazilian propolis seemed to be more efficient than that from Bulgaria, because of their different chemical composition. In Bulgaria, bees collect the material mainly from the bud exudate of poplar trees, while in Brazil, Baccharis dracunculifolia DC. was shown to be the main propolis source. Our data also showed that the increased bactericidal activity of macrophages involved the participation of oxygen (H(2)O(2)) and nitrogen (NO) intermediate metabolites.
DOI: 10.1016/j.intimp.2004.10.003
PubMed: 15652765
Affiliations:
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Macrophages play an important role in the early phase of Salmonella infection. In this work, macrophages were prestimulated with Brazilian or Bulgarian propolis and subsequently challenged with Salmonella Typhimurium at different macrophage/bacteria ratio. After 60 min of incubation, cells were harvested with Triton-X to lyse the macrophages. To assess the bactericidal activity, the number of colony-forming units (CFU) of S. typhimurium was determined by plating 0.1 mL in Mueller Hinton agar. After 24 h, CFU were counted, and the percentage of bactericidal activity was obtained. Propolis from Brazil and Bulgaria enhanced the bactericidal activity of macrophages, depending on its concentration. Brazilian propolis seemed to be more efficient than that from Bulgaria, because of their different chemical composition. In Bulgaria, bees collect the material mainly from the bud exudate of poplar trees, while in Brazil, Baccharis dracunculifolia DC. was shown to be the main propolis source. Our data also showed that the increased bactericidal activity of macrophages involved the participation of oxygen (H(2)O(2)) and nitrogen (NO) intermediate metabolites.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15652765</PMID><DateCompleted><Year>2005</Year><Month>06</Month><Day>28</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1567-5769</ISSN><JournalIssue CitedMedium="Print"><Volume>5</Volume><Issue>2</Issue><PubDate><Year>2005</Year><Month>Feb</Month></PubDate></JournalIssue><Title>International immunopharmacology</Title><ISOAbbreviation>Int Immunopharmacol</ISOAbbreviation></Journal><ArticleTitle>Effects of Brazilian and Bulgarian propolis on bactericidal activity of macrophages against Salmonella Typhimurium.</ArticleTitle><Pagination><MedlinePgn>359-68</MedlinePgn></Pagination><Abstract><AbstractText>Propolis has been used in folk medicine since ancient times due to its many biological properties, such as antimicrobial, antiinflammatory, antioxidant, immunomodulatory activities, among others. Macrophages play an important role in the early phase of Salmonella infection. In this work, macrophages were prestimulated with Brazilian or Bulgarian propolis and subsequently challenged with Salmonella Typhimurium at different macrophage/bacteria ratio. After 60 min of incubation, cells were harvested with Triton-X to lyse the macrophages. To assess the bactericidal activity, the number of colony-forming units (CFU) of S. typhimurium was determined by plating 0.1 mL in Mueller Hinton agar. After 24 h, CFU were counted, and the percentage of bactericidal activity was obtained. Propolis from Brazil and Bulgaria enhanced the bactericidal activity of macrophages, depending on its concentration. Brazilian propolis seemed to be more efficient than that from Bulgaria, because of their different chemical composition. In Bulgaria, bees collect the material mainly from the bud exudate of poplar trees, while in Brazil, Baccharis dracunculifolia DC. was shown to be the main propolis source. Our data also showed that the increased bactericidal activity of macrophages involved the participation of oxygen (H(2)O(2)) and nitrogen (NO) intermediate metabolites.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Orsi</LastName><ForeName>Ricardo O</ForeName><Initials>RO</Initials><AffiliationInfo><Affiliation>Department of Production and Animal Exploration-School of Veterinary Medicine and Animal Husbandry-UNESP, 18618-000 Botucatu, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sforcin</LastName><ForeName>José M</ForeName><Initials>JM</Initials></Author><Author ValidYN="Y"><LastName>Funari</LastName><ForeName>Sílvia R C</ForeName><Initials>SR</Initials></Author><Author ValidYN="Y"><LastName>Bankova</LastName><ForeName>Vassya</ForeName><Initials>V</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Int Immunopharmacol</MedlineTA><NlmUniqueID>100965259</NlmUniqueID><ISSNLinking>1567-5769</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007155">Immunologic Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>9009-62-5</RegistryNumber><NameOfSubstance UI="D011429">Propolis</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001938" MajorTopicYN="N" Type="Geographic">Brazil</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002031" MajorTopicYN="N" Type="Geographic">Bulgaria</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007155" MajorTopicYN="N">Immunologic Factors</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008262" MajorTopicYN="N">Macrophage Activation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017737" MajorTopicYN="N">Macrophages, Peritoneal</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011429" MajorTopicYN="N">Propolis</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012486" MajorTopicYN="Y">Salmonella typhimurium</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>08</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2004</Year><Month>09</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2004</Year><Month>10</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>1</Month><Day>18</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>6</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>1</Month><Day>18</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15652765</ArticleId><ArticleId IdType="pii">S1567-5769(04)00341-8</ArticleId><ArticleId IdType="doi">10.1016/j.intimp.2004.10.003</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region></list><tree><noCountry><name sortKey="Bankova, Vassya" sort="Bankova, Vassya" uniqKey="Bankova V" first="Vassya" last="Bankova">Vassya Bankova</name><name sortKey="Funari, Silvia R C" sort="Funari, Silvia R C" uniqKey="Funari S" first="Sílvia R C" last="Funari">Sílvia R C. Funari</name><name sortKey="Sforcin, Jose M" sort="Sforcin, Jose M" uniqKey="Sforcin J" first="José M" last="Sforcin">José M. Sforcin</name></noCountry><country name="Brésil"><region name="État de São Paulo"><name sortKey="Orsi, Ricardo O" sort="Orsi, Ricardo O" uniqKey="Orsi R" first="Ricardo O" last="Orsi">Ricardo O. Orsi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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